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公司与广州健康与生命学院联合研究成果发表在《Nature Methods》上
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近日,《Nature Methods》发表了由我公司技术总监刘骁博士与中科院广州健康与生命学院再生生物学重点实验室包教授课题组联合署名的文章“Capturing the interactome of newly transcribed RNA”。文章介绍了对一种新的RNA结合蛋白检测技术RICK(capture of the newly transcribed RNA interactome using click chemistry)的相关研究成果,摘要原文如下:


We combine the labeling of newly transcribed RN As with 5-ethynyluridine with the characterization of bound proteins.This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematicallycaptures proteins bound to a wide range of RN As, including nascent RN As and traditionally neglected nonpolyadenylated RNAs. RICK has identified mitotic regulators amongst other novel RNA binding proteins with preferential affinity for nonpolyadenylated RNAs, revealed a link between metabolic enzymes/factors and nascent RNAs, and expanded the known RNA-bound proteome of mouse embryonic stem cells. RIC K will facilitate an in-depth interrogation of the total RNA-bound proteome in different cells and systems.